Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis

Asthma is one of the most common noninfectious chronic diseases characterized by type II inflammation. This study aimed to investigate effects molecular hydrogen on pathogenesis asthma. OVA sensitized asthma mouse model and house dust mite treated 16HBE cellular were established hydrogen/oxygen mixture was used treat asthmatic mice cells. Serum BALF cytokines measured with specific ELISA assays. E-cadherin ZO-1 detected immunohistochemical staining expression caspase 3 9, NF-κB, IL-33 ST2 assessed quantitative real-time PCR, western blot and/or immunofluorescence. promoter activity analyzed dual-luciferase assay. ILC2 population assayed flow cytometry differentially expressed miRNAs using miRNA array. levels other alarmin greatly increased inhibited H2 in mice. The NF-κB (p65) upregulated suppressed H2. markedly OVA-induced mice, such increase airway tissues significantly lower than that control reduction recovered treatment. alleviated HDM induced apoptosis cells, upregulation ST2, elevation activity. A group + cells identified. These data demonstrated efficient suppressing allergen-induced could be developed as a therapeutics for conditions